NanoDiagnostics QC
Cat. No.RN-CY-EL-003
NO Human IgG Fc Lateral Flow Dipstick Assay Kit
Product overview: Cytodiagnostics Human IgG ELISA kit is a sensitive and accurate assay for the quantitative determination of human IgG levels produced in vivo and for assessing the level of immunoglobulin secretion by a hybridoma in vitro. The Human IgG ELISA kit is based on the antibody sandwich principle. A microtiter plate coated with a capture antibody specific to human IgG Fc has been blocked and stabilized to create the solid phase of the assay. To perform the assay, samples, standards, and controls are added directly to the wells of the plate. After washing away unbound IgG, an HRP-conjugated Detection Antibody Solution is added and binds to the heavy and light chains of the captured human IgG protein that were immobilized by the capture antibody, completing the sandwich. The wells are washed and a tetramethylbenzidine (TMB) Substrate Solution is added. A blue colour develops in proportion to the amount of bound human IgG. The color development is stopped using Stop Solution, which turns the blue end-product to yellow and the optical density (OD) of the yellow product is measured at 450 nm on a microtiter plate reader. Our ELISA kit has several benefits: Ready to use. Simply add standards and samples directly to wells. No need for lengthy blocking or rehydration steps. High sensitivity, specificity and reproducibility. Fully validated in the sample types listed below. 96-well plate is breakable into 12 x 8 well strips.
Key Specs
Catalog No.
RN-CY-EL-003
CAS Number
Procurement Snapshot
MOQ
1
Lead Time
On request
Availability
Global
Shipping
Standard
Storage
Refer TDS/SDS
Shelf Life
Refer TDS
Grade
Research
Batch Docs
On request
✓Use For
- Application guidance is available from the Reinste technical team.
- R&D and industrial evaluation workflows.
⚠️Not Recommended For
- Review application suitability with technical support before use.
- Follow the storage and handling instructions provided for this product.
Variant Matrix
| Pack Size | Purity | Price | Stock |
|---|---|---|---|
| 1 | On request | Enquire | On request |
Overview
Product overview: Cytodiagnostics Human IgG ELISA kit is a sensitive and accurate assay for the quantitative determination of human IgG levels produced in vivo and for assessing the level of immunoglobulin secretion by a hybridoma in vitro. The Human IgG ELISA kit is based on the antibody sandwich principle. A microtiter plate coated with a capture antibody specific to human IgG Fc has been blocked and stabilized to create the solid phase of the assay. To perform the assay, samples, standards, and controls are added directly to the wells of the plate. After washing away unbound IgG, an HRP-conjugated Detection Antibody Solution is added and binds to the heavy and light chains of the captured human IgG protein that were immobilized by the capture antibody, completing the sandwich. The wells are washed and a tetramethylbenzidine (TMB) Substrate Solution is added. A blue colour develops in proportion to the amount of bound human IgG. The color development is stopped using Stop Solution, which turns the blue end-product to yellow and the optical density (OD) of the yellow product is measured at 450 nm on a microtiter plate reader. Our ELISA kit has several benefits: Ready to use. Simply add standards and samples directly to wells. No need for lengthy blocking or rehydration steps. High sensitivity, specificity and reproducibility. Fully validated in the sample types listed below. 96-well plate is breakable into 12 x 8 well strips.
Applications
- Application guidance is available from the Reinste technical team.
Storage & Handling
- Store as per product document and SDS guidance.
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